A sensitive, specific, precise and accurate high performance liquid chromatographic method of analysis of a mucolytic drug Erdosteine was developed and validated. The method employed in Kromasil ODS C18 (100 x 4.6 mm 5.0�µ) column as a stationary phase. The mobile phase contains a mixture of Acetonitrile, Phosphate buffer pH adjusted to 2.5 using phosphoric acid and Triethylamine. It is pumped isocratically through the chromatographic system at a flow rate of 1.0 ml minute. UV detector was operated at 236 nm. The analyte peak was eluted at retention time of 3.02 minutes .The method was found to be linear over a range of 2- 10 �µg/ml. The developed Method was validated as per ICH guidelines. The developed method is thus having a thorough output in the estimation of Erdosteine in bulk drug.
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